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[Fifth AACR International Conference on Frontiers in Cancer Prevention Research, Nov 12-15, 2006]


Cell, Molecular, and Tumor Biology: Viral Oncogenesis and Mechanisms

Human papillomavirus (HPV)-associated carcinogenesis: Identification of putative cellular target genes by RNA interference affecting viral oncogene expression and subsequent transcriptome analysis.

Ruprecht A. Kuner, Markus Vogt, Mark Fellmann, Karin Butz, Andreas Buness, Annemarie Poustka, Felix Hoppe-Seyler and Holger Sültmann

German Cancer Research Center, Heidelberg, Germany

Abstract

A138

Specific types of human papillomaviruses (HPVs) cause cervical cancer, the second most common tumor in females worldwide. Cellular transformation is linked to expression of the viral E6 and E7 oncogenes. The E6 oncoprotein induces the proteolytic degradation of p53, whereas E7 inactivates the retinoblastoma protein, pRb, and other pocket proteins. We employed the RNA interference (RNAi) technology, in order to inhibit E6 expression alone, or in combination with E7, in HPV-positive HeLa cells. After genome-wide microarray experiments, the set of genes which was affected by RNAi was compared with gene expression data from p53 siRNA-knockdown experiments and with public available from HPV-positive cervical carcinoma tissue. In this meta-analysis, a large accordance was found between the genes affected in our E6/E7 knockdown experiments and genes which were found to be modulated in HPV-positive cervical cancer biopsies. After functional annotation, we were able to confirm the affection of known tumor-relevant processes, like the inhibition of cell cycle arrest and apoptosis, but found also novel molecular aspects of HPV-driven tumorigenesis.







HOME HELP FEEDBACK HOW TO CITE ABSTRACTS ARCHIVE CME INFORMATION SEARCH
Cancer ResearchClinical Cancer Research
Cancer Epidemiology Biomarkers & PreventionMolecular Cancer Therapeutics
Molecular Cancer ResearchCancer Prevention Research
Cancer Prevention Journals PortalCancer Reviews Online
Annual Meeting Education BookMeeting Abstracts Online
Copyright © 2006 by the American Association for Cancer Research.